Dr YEUNG, Man Lung Philip

 

BSc, PhD

 

Assistant Professor, Department of Microbiology


Email: pmlyeung@hku.hk

 

ORCID : https://orcid.org/0000-0002-6117-7884

 

 

Research Interests

» Studying host-virus interactions using state-of-the-art technologies, including high-throughput sequencing, genome editing (CRISPR-Cas/TALEN) and genome-wide library scale RNA interference (RNAi) systems.

» Molecular pathogenesis of respiratory and enteric viruses, including Middle East respiratory syndrome coronavirus (MERS-CoV), influenza A virus and Enterovirus 71 (EV71).

» Studying regulatory roles of small RNAs derived from host and virus on virus replication and host response.

 

Selected Publications

Yeung ML, Tam TS, Tsang AC, Yao KM. Proteolytic cleavage of PDZD2 generates a secreted peptide containing two PDZ domains. Embo Rep 2003; 4: 412-8.

Bennasser Y, Le SY, Yeung ML, Jeang KT. HIV-1 encoded candidate micro-RNAs and their cellular targets. Retrovirology 2004; 1: 43.

Yeung ML, Bennasser Y, Myers TG, Jiang G, Benkirane M, Jeang KT. Changes in microRNA expression profiles in HIV-1-transfected human cells. Retrovirology 2005; 2: 81.

Bennasser Y, Yeung ML, Jeang KT. HIV-1 TAR RNA subverts RNA interference in transfected cells through sequestration of TAR RNA-binding protein, TRBP. J Biol Chem 2006; 281: 27674-8.

Hai T, Yeung ML, Wood TG, Boldogh I, Wei Y, Yamaoka S, Gatalica Z, Jeang KT, and Brasier AR. An alternative splice product of IkappaB kinase (IKKgamma), IKKgamma-delta, differentially mediates cytokine and human T-cell leukemia virus type 1 tax-induced NF-kappaB activation. J Virol 2006; 80: 4227-41.

Ma RY, Tam TS, Suen AP, Yeung ML, Tsang SW, Chung SK, Thomas MK, Leung PS and Yao KM. Secreted PDZD2 exerts concentration-dependent effects on the proliferation of INS-1E cells. Int J Biochem Cell Biol 2006; 38: 1015-22.

Gironella M, Seux M, Xie MJ, Cano C, Tomasini R, Gommeaux J, Garcia S, Nowak J, Yeung ML, Jeang KT, Chaix A, Fazli L, Motoo Y, Wang Q, Rocchi P, Russo A, Gleave M, Dagorn JC, Iovanna JL, Carrier A, Pébusque MJ, Dusetti NJ. Tumor protein 53-induced nuclear protein 1 expression is repressed by miR-155, and its restoration inhibits pancreatic tumor development. Proc Natl Acad Sci U S A 2007; 104: 16170-5.

Houzet L*, Yeung ML*, de Lame V, Desai D, Smith SM, Jeang KT. MicroRNA profile changes in human immunodeficiency virus type 1 (HIV-1) seropositive individuals. Retrovirology 2008; 5: 118.

Watashi K, Khan M, Yedavalli VR, Yeung ML, Strebel K, Jeang KT. Human immunodeficiency virus type 1 replication and regulation of APOBEC3G by peptidyl prolyl isomerase Pin1. J Virol 2008; 82: 9928-36.

Yeung ML, Yasunaga J, Bennasser Y, Nelson D, Harris D, Ahmad N, Matsuoka M and Jeang KT. Roles for microRNAs, miR-93 and miR-130b, and tumor protein 53-induced nuclear protein 1 tumor suppressor in cell growth dysregulation by human T-cell lymphotrophic virus 1. Cancer Res 2008; 68: 8976-85.

Yeung ML, Bennasser Y, Watashi K, Le SY, Houzet L, Jeang KT. Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid. Nucleic Acids Res 2009; 37: 6575-86.

Yeung ML, Houzet L, Yedavalli VS, Jeang KT. A genome-wide short hairpin RNA screening of jurkat T-cells for human proteins contributing to productive HIV-1 replication. J Biol Chem 2009; 284: 19463-73.

Sanville B, Dolan MA, Wollenberg K, Yan Y, Martin C, Yeung ML, Buckler-White A and Kozak CA. Adaptive evolution of Mus Apobec3 includes retroviral insertion and positive selection at two clusters of residues flanking the substrate groove. Plos Pathog 2010; 6: e1000974.

Watashi K, Yeung ML, Starost MF, Hosmane RS, Jeang KT. Identification of small molecules that suppress microRNA function and reverse tumorigenesis. J Biol Chem 2010; 285: 24707-16.

Banaudha K, Kaliszewski M, Korolnek T, Florea L, Yeung ML, Jeang KT, Kumar A. MicroRNA silencing of tumor suppressor DLC-1 promotes efficient hepatitis C virus replication in primary human hepatocytes. Hepatology 2010; Jan;53(1):53-61.

Woo PC, Lau SK, Choi GK, Huang Y, Teng JL, Tsoi HW, Tse H, Yeung ML, Chan KH, Jin DY, Yuen KY. Natural occurrence and characterization of two IRES elements in a novel virus, canine picodicistrovirus, in the picornavirus-like superfamily. J Virol. 2012 Mar;86(5):2797-808.

Houzet L, Klase Z, Yeung ML, Wu A, Le SY, Quiñones M, Jeang KT. The extent of sequence complementarity correlates with the potency of cellular miRNA-mediated restriction of HIV-1. Nucleic Acids Res. 2012 Dec;40(22):11684-96.

Chan JF, Chan KH, Choi GK, To KK, Tse H, Cai JP, Yeung ML, Cheng VC, Chen H, Che XY, Lau SK, Woo PC, Yuen KY. Differential Cell Line Susceptibility to the Emerging Novel Human Betacoronavirus 2c EMC/2012: Implications for Disease Pathogenesis and Clinical Manifestation. J Infect Dis. 2013 Jun 1;207(11):1743-52.

Siu KL*, Yeung ML*, Kok KH*, Yuen KS, Kew C, Lui PY, Chan CP, Tse H, Woo PCY, Yuen KY, Jin DY. Middle East respiratory syndrome coronavirus 4a protein suppresses type I interferon production by preventing PACT-induced activation of RIG-I and MDA5. J Virol. 2014 May;88(9):4866-76.

Chan JF, Choi GK, Tsang AK, Tee KM, Lam HY, Yip CC, To KK, Cheng VC, Yeung ML, Lau SK, Woo PC, Chan KH, Tang BS, Yuen KY. Development and evaluation of novel real-time RT-PCR assays with locked nucleic acid probes targeting the leader sequences of human pathogenic coronaviruses. J Clin Microbiol. 2015 Aug;53(8):2722-6.

Chu H, Zhou J, Wong BH, Li C, Chan JF, Cheng ZS, Yang D, Wang D, Lee AC, Li C, Yeung ML, Cai JP, Chan IH, Ho WK, To KK, Zheng BJ, Yao Y, Qin C, Yuen KY. Middle East respiratory syndrome coronavirus efficiently infects human primary T lymphocytes and activates both the extrinsic and intrinsic caspase-dependent apoptosis pathways. J Infect Dis. J Infect Dis. 2015 Jul 22. pii: jiv380.

Chan JF, Yao Y, Yeung ML, Deng W, Bao L, Jia L, Li F, Xiao C, Gao H, Yu P, Chu H, Cai JP, Zhou J, Chen H, Qin C and Yuen KY. Treatment with lopinavir/ritonavir or interferon-β1b improves outcome of MERS-CoV infection in a non-human primate model of common marmoset. J Infect Dis. J Infect Dis. 2015 Dec 15;212(12):1904-13. doi: 10.1093/infdis/jiv392.

Lui PY, Wong LY, Fung CL, Siu KL, Yeung ML, Yuen KS, Chan CP, Woo PC, Yuen KY, Jin DY. Middle East respiratory syndrome coronavirus M protein suppresses type I interferon expression through the inhibition of TBK1-dependent phosphorylation of IRF3. Emerging microbes & infections 5, e39 (2016).

Yeung ML*, Yao Y*, Jia L, Chan JF, Chan KH, Cheung KF, Chen H, Poon VK, Tsang AK, To KK, Yiu MK, Teng JL, Chu H, Zhou J, Zhang Q, Deng W, Lau SK, Lau JY, Woo PC, Chan TM, Yung S, Zheng BJ, Jin DY, Mathieson PW, Qin C and Yuen KY. Middle East respiratory syndrome coronavirus induces apoptosis in kidney and lung through upregulation of Smad7 and FGF2. Nat Microbiol. 2016 1, 16004.

Yeung ML, Jia L, Yip CC, Chan JF, Teng JL, Chan KH, Cai JP, Zhang C, Zhang AJ, Wong WM, Kok KH, Lau SK, Woo PC, Lo JY, Jin DY, Shih SR and Yuen KY. Human tryptophanyl-tRNA synthetase is an IFN-gamma-inducible entry factor for Enterovirus. J Clin Invest. 2018 Aug 28. pii: 99411.

* Equal contribution

  

Editorship:

Academic Editor, PLoS ONE (2010 - present)